The rat IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Ralpha chain (CD126), and the signal-transducing component gp130 (also called CD130). The rat IL-6 shares 40 % and 85 % aa sequence identity with human and mouse IL-6 and it is equally active on human.
R-PLEX Rat IL-6 Antibody Set Overview The R-PLEX Rat IL-6 Antibody Set includes a matched capture and detection antibody pair and calibrator for the quick and easy measurement of IL-6 in biological samples. R-PLEX products leverage MSD’s electrochemiluminescence technology for better sensitivity and a larger dynamic range than traditional ELISAs.
Rat IL-6 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat IL-6 in the samples. A monoclonal antibody specific for rat IL-6 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-6, and IL-6 present is bound by.
The cytokine interleukin 6 (IL-6), a major mediator of immune and acute phase responses of the liver, has been implicated in the termination of pregnancy once expressed in the uterus. This study was undertaken to investigate the expression and regulation of genes encoding IL-6 and IL-6 receptor (IL-6R) in rat decidual tissue. Total RNA obtained from rat decidual tissue on different days of.
IL6 (Interleukin 6) is a Protein Coding gene. Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.Among its related pathways are Th1 Differentiation Pathway and Hematopoietic cell lineage.Gene Ontology (GO) annotations related to this gene include signaling receptor binding and growth factor activity.
The Quantikine Rat IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results.
The Rat Genome Database (RGD) is the premiere location for rat genomics, genetics, physiology and functional data, as well as data for comparative genomics between rat, human and mouse. RGD is responsible for attaching biological information to the rat genome via structured vocabulary, or ontology, annotations assigned to genes and quantitative trait loci (QTL), and for consolidating rat.
This Rat IL-6 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat IL-6 in the sample, this Rat IL-6 ELISA Kit includes a set of calibration standards. The.
Interleukin-6 (IL-6) is a cytokine that is expressed by T cells, macrophages, and muscle cells. IL-6 acts to stimulate an immune response upon infection or trauma. IL-6 has both pro-inflammatory and anti-inflammatory functions and signals through the cell-surface type 1 cytokine receptor complex containing the IL-6Ra and gp130 chains. Human IL-6 is active on mouse and rat cells.
Neutralisation of endogenous IL-6 with a sheep anti-rat IL-6 serum did not alter heat-induced CGRP release, but abolished the sIL-6R-mediated sensitising effect. We show that IL-6 in combination with its soluble receptor can sensitise nociceptors to heat and provide evidence for the constitutive expression of the signalling molecule gp130, but not of the IL-6-membrane-bound (specific) receptor.
Interleukin-6 Rat Recombinant. Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. The IL6 is purified by proprietary chromatographic techniques.
Intended Uses: The rat IL-6 ELISA has been designed for the precise analytical measurement of IL-6 concentrations in serum, body fluids, tissue homogenate or cell culture supernates from rat. For research use only, not for use in diagnostic procedures. Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody.
Recombinant Rat Interleukin 6. Products. Human Primary Cell Culture. Airway Epithelial Cells; Cardiac Myocytes.
Rat IL6 ELISA Kits The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers and can help streamline your immunodetection experiments.
IL-6 originates from mononuclear phagocytes, T and B lymphocytes, vascular endothelial cells and fibroblasts. It has a role in various physiological and pathological processes. IL-6 plays an important role in intracellular signaling pathways. Recombinant rat IL-6 is a 21.7kDa protein containing 187 amino acid residues. Physical form.
Rat IL-6 ELISA KIT 1. Intended use The rat IL-6 ELISA is an enzyme-linked immunosorbent assay for quantitative detection of rat IL-6 in cell culture supernatants or rat serum. This kit has been configured for research use only. Not suitable for use in therapeutic procedures. 2. Introduction 2.1. Summary Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute.
Interleukin 6 (IL-6) is a cytokine that is expressed by T cells, macrophages, and muscle cells. IL-6 acts to stimulate an immune response upon infection or trauma. IL-6 has both pro-inflammatory and anti-inflammatory functions, is capable of promoting fever, and signals through the cell-surface type I cytokine receptor complex containing the IL-6Ra and gp130 chains.
IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis. Description Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton.
IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through.